Journal: Journal of Neuroinflammation
Article Title: Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury
doi: 10.1186/s12974-023-02903-w
Figure Lengend Snippet: PAD4 promotes neuroinflammation and neuronal death via IRE1α/ASK1/JNK signaling pathway after TBI. A Schematic image of adeno-PAD4-EGFP and overexpression of PAD4 in the cortex of the mouse. B , C mNSS test on day 1 ( B ) and days 3 ( C ) post-TBI. **p < 0.01, ***p < 0.001, n = 10 per group. D , E Rotarod test on day 1 ( D ) and days 3 ( E ) post-TBI. *p < 0.05, **p < 0.01, ***p < 0.001, n = 10 per group. F Representative western blot bands and densitometric quantification of Iba-1, CD16, and CD206 in the contused cortex after TBI. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6 per group. G Representative image of the FJC (green) staining and representative images of TUNEL (green) co-localization with neurons (NeuN, red) in the contused cortex at 3 days after TBI. Nuclei were stained with DAPI (blue). Scale bar = 100 μm. H , I Quantitative analysis of FJC-positive cells ( H ) and quantitative analysis of TUNEL-positive neurons ( I ) in the contused cortex at 3 days after TBI. *p < 0.05, **p < 0.01, n = 6 per group. J Representative western blot bands and densitometric quantification of pIRE1α, pASK1, and pJNK in the contused cortex after TBI. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6 per group
Article Snippet: Recombinant PAD4 adenovirus (Ad-PAD4; Adeno-CMV-MCS-Padi4-3FLAG- SV40-EGFP, serotype Ad5, 4 × 10 10 plaque-forming-unit/mL) and empty control adenovirus (Ad-con, Adeno-CMV-MCS-3-FLAG-SV40-EGFP) were produced by Genechem (Shanghai, China).
Techniques: Over Expression, Western Blot, Staining, TUNEL Assay
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